Solid phase extraction (SPE) is an extraction method that uses a solid phase and a liquid phase to .. more tortuous path of flow, which means that there is con-. Solid-phase extraction is designed for rapid sample preparation and purification prior to chromatographic analysis. SPE is widely used in analytical laboratories. The solid phase extraction (SPE) is a pre-processing technique commonly used in laboratories for the extraction of analytes from complex matrices.
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What is Solid Phase Extraction (SPE) ?
This affinity allows the separation of the target analyte from the interferents. A typical solid phase extraction involves four steps: First, the cartridge is equilibrated or conditioned with a solvent to wet the sorbent.
Then the loading solution containing the analyte is solid phase extraction definition through the solid phase. Ideally, the analyte and some impurities are retained on the sorbent. Once the charge is neutralized, the electrostatic interaction between the analyte and the stationary phase no longer exists and the analyte will elute from the cartridge.
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Strong cation exchange sorbents contain aliphatic sulfonic acid groups that are always negatively charged in aqueous solution, and weak cation exchange sorbents contain aliphatic solid phase extraction definition acids, which are charged when the pH is above about 5.
Strong cation exchange sorbents are useful because any strongly basic impurities in the sample will bind to the sorbent and usually will not be eluted with the analyte of interest; to recover a strong base a weak cation exchange cartridge should be used. To elute the analyte from either the strong or weak sorbent, the stationary phase is washed solid phase extraction definition a solvent that neutralizes ionic interaction between the analyte and the stationary phase.
The manifold allows multiple samples to be processed by holding several SPE media in place and allowing for an equal number of samples to pass through them simultaneously.
Solid phase extraction - Wikipedia
In a standard cartridge SPE manifold up to 24 cartridges can be mounted in parallel, while a typical disk SPE manifold can accommodate 6 disks. Most Solid phase extraction definition manifolds are equipped with a vacuum port, where vacuum can be applied to speed up the extraction process by pulling the liquid sample through the stationary phase.
The analytes are collected in sample tubes inside or below the manifold after they pass through the stationary phase. Solid phase extraction cartridges and disks are available with a variety of stationary phases, each of which can separate analytes according to different chemical properties.
Most stationary phases are based on silica that has been bonded to a specific functional group. The general procedure is to load a solution onto the Solid phase extraction definition phase, wash away undesired components, and then wash off the desired analytes with another solvent into a collection tube.
Instrumention Solid-phase extractions use the same type of stationary phases as are used in liquid chromatography columns. The stationary phase is contained in a glass or plastic column above a frit or glass wool.