Saprolegnia species affect to decline of the wild fish [2] and cause diseases in .. Also it is reported that Saprolegnia sp. infection can occur in. Saprolegnia is a genus of water moulds often called cotton moulds because of the characteristic white or grey fibrous patches they form. Current taxonomy puts  Phylum‎: ‎Heterokontophyta. Our study revealed the presence of Saprolegnia sp. in the Andean frog Atelopus mittermeieri using the imprinting technique with lactophenol blue staining.


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Characterisation of Saprolegnia sp. isolates from channel catfish.

Lawsone 2-hydroxy-1,4-naphtoquinone is the principal active component in extract of henna Lawsonia inermis leaves [ 18 ], and exhibits wide range of bioactivities such as antibacterial, antifungal, antimalarial, antitumor, and antioxidant [ 19 ].

Therefore, we investigated the efficacy of extracted lawsone against isolated S. Zebrafish Danio rario is considered as powerful animal model widely used saprolegnia sp many research fields including various infection models upon bacteria [ 20 ], virus [ 21 ], yeast [ 22 ], and saprolegnia sp infection [ 23 ].

Most of the studies related to pathogenicity and host pathogen interactions of S. Saprolegnia sp, we initiated fundamental investigations for developing zebrafish disease model for S.

In this study, morphological and molecular identification were conducted for the isolated S. Furthermore, plant based natural lawsone was tested against isolated S.

The saprolegnia sp mode of action of lawsone on S. To isolate and identify pathogen s associated with the disease, skin tissue samples were collected and repeated sub-culturing was performed.

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To obtain pure culture, sub-culturing was performed for 5 times by cutting and transferring a saprolegnia sp section of growing margin of mycelium into new plate by using a sterile surgical blade.

Morphological and molecular identification Morphological features and molecular based saprolegnia sp were applied to identify the isolated pathogen. Gross and microscopic features of radial growth of pathogen were observed in PDA plate. To identify zoospore as asexual reproduction, the margin of advancing mycelium was collected using 0.

  • Characterisation of Saprolegnia sp. isolates from channel catfish.
  • Saprolegnia - Wikipedia

After 24 saprolegnia sp, mycelium was collected and washed twice using sterilized tap water STW and incubated for another 24hr in STW. Then mature sporangium and zoospores were observed through light microscope.

Additionally, secondary zoospores were observed saprolegnia sp stained with standard lactophenol cotton blue wet mount preparation as described by Sime et al. Phylogenetic analysis was conducted using 22 counterparts of Saprolegnia species and other saprolegnia sp oomycete strains to understand the taxonomic relationship of isolated S.

To understand the pathogenicity and host pathogen interactions zebrafish embryo, juvenile and adult were saprolegnia sp to secondary zoospores of S. Firstly, to observe the survival rate and hatching delay, zebrafish eggs were obtained from adult AB line zebrafish.

In each group, 10 eggs were transferred into petri dish containing egg water saprolegnia sp 6 replicates. During these experiments, same conditions were maintained for the control group without zoospore exposure.


Survival saprolegnia sp and other morphological features of embryo, and juveniles were observed for saprolegnia sp days at every hr intervals. To detect Saprolegnia sp. A preparation of hemolymph HL from adult female of D.

The hemolymph response, against the presence of the oomycete in vitro, was measured spectrophotometrically at nm.

The saprolegnia sp strain of Saprolegnia sp. The strain of Saprolegnia sp. There is important to have quick methods to detect and prevent infections avoiding massive loses of fish production.

Protist Images: Saprolegnia

In this work we propose a tool for its detection that does not require expensive material for the application. Keywords Carminic acid; Saprolegniasis; Granulocytes; Carassius auratus Introduction Saprolegnia is an oomycete that has long been considered as a separate class within the saprolegnia sp Fungi.

Saprolegnia sp, sequence DNA comparisons indicate that they are closer to brown algae, with a worldwide distribution [ 12 ]. The growth sign of the disease typically is manifested as a relativity superficial, cotton-wool like, white growth of mycelia on the fish skin specially around the head, dorsal and caudal fins, gills, in muscular layer and internal organs [ 3 - 5 ].

Saprolegnia sp though Saprolegnia sp.