POLYKETIDE SYNTHASE PDF

Polyketide synthases (PKSs) are structurally and functionally related to fatty acid synthases (FAS's), as both enzyme classes catalyzes the. Type III polyketide synthases (PKSs) are simple homodimers of ketosynthases which catalyze the condensation of one to several molecules of extender. Type III polyketide synthases include plant naringenin-chalcone synthases (CHSs) [PMID: ] and stilbene synthases (STSs) (resveratrol synthases).


POLYKETIDE SYNTHASE PDF

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POLYKETIDE SYNTHASE PDF


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POLYKETIDE SYNTHASE PDF

In conjunction with polyketide synthase Department of Biochemistry DNA Sequencing Facility, we recently published the entire sequence of Saccharopolyspora erythraeaan erythromycin-producing bacterium.

First polyketide synthase starter unit, in the form of an acetyl, is loaded into the b-ketosynthase domain KS of the enzyme, a process that is mediated by the acyl-carrier-protein domain ACP step 1 in figure 5.

POLYKETIDE SYNTHASE PDF

The acetyl is delivered to the polyketide synthase in the form of acetyl-CoA and polyketide synthase to the enzyme via a thioester bond Nelson and Cox, The extender unit is typically a malonyl delivered to the enzyme by CoA.

The KS domain catalyze the Claisen condensation reaction between the starter and extender units, driven by decarboxylation of the extender unit Proctor et al. At this point two different options exist: In option 1 polyketide synthase product is transferred back to the KS domain to prepare for a second iteration step 9 in figure 5 and another extender unit is loaded into the enzyme.

POLYKETIDE SYNTHASE PDF

For option 2 to occur the polyketide have to have reached its predetermined length which is unique polyketide synthase each iPKS.

The polyketide is transferred to the thioesterase polyketide synthase TE that catalyzes its release from the enzyme Hendrickson et al.

Their structures are assembled by polyketide synthases Polyketide synthase. Modular PKSs are composed of modules, which involve sets of domains catalysing the stepwise polyketide biosynthesis.

The polyketide chain is handed over from the ACP domain of the previous module to the KS domain of the current module, catalyzed by the KS domain.

The reaction takes polyketide synthase at the KSn-bound end of the polyketide synthase, so that the chain moves out one position and the elongation group becomes the new bound group.