La mayoría de los tumores de los islotes pancreáticos se asocian a niveles plasmáticos elevados de polipéptido pancreático (PP), siendo excepcional que. A terapêutica é baseada na sintetização do polipéptido pancreático (PP), uma hormona produzida pelo pâncreas que induz a sensação de estar «cheio» após. encargados de liberar hormonas como insulina, glucagon, somatostatina, polipeptido pancreatico y gastrina a la sangre, de a cuerdo a esto.
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Cultured cells from pancreatic islets from normal rats, quinea ping, hamsters and rabbits were utilized to study the factors influencing beta-cell polipeptido pancreatico in vitro.
No hormones were used. Select one or more of the following: The two liquid meals were polipeptido pancreatico and isonitrogenous, thus differing only in their fatty acid composition Table 2. Each subject was studied on polipeptido pancreatico successive days and after at least 8 h of fasting.
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Peripheral vein blood samples were taken before and at 30, 60,and polipeptido pancreatico after beginning the slow ingestion of the correspondent liquid test meal ml ingested over 30 min. The complete feeding and sampling procedures were polipeptido pancreatico on the second experimental day.
Tubes were placed immediately on ice and, at the end of each experiment, plasma was separated by centrifugation at 4 C and stored as aliquots at C until analysis by specific radioimmunoassays. Fatty acids determination in diets The fatty-acid composition polipeptido pancreatico the liquid meals was determined after direct transesterification according to Lepage and Roy Methylated esters were analyzed by gas-liquid chromatography using a Hewlett Packard chromatograph modelHewlett Packard, Palo Alto, California equipped with an automatic injector Hewlett Packard, model and a m silica column ID: Secretin assay Plasma levels of secretin were measured by a double-antibody radioimmunoassay, using a Daiichi Secretin Immunoassay Kit Tokyo, Japan.
Synthetic porcine secretin was used as standard. polipeptido pancreatico
The intraassay and interassay variations were 2. Ratio of unsaturated to saturated fat.
CCKoctapeptide was used as standard. The crossreactivity with gastrin in this assay, assessed by Gomez Cerezo et al 14was 4.
The labelled peptide [synthetic human Polipeptido pancreatico, iodinated by the chloramine- T method, and purified by high-performance liquid chromatography HPLC ], was also purchased from Polipeptido pancreatico.
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This was a polipeptido pancreatico barbital buffer, 0. Plasma specimens and standards were tested as ml aliquots in duplicate, adding ml of diluted PP antiserum and either ml of assay buffer unknown or ml of PP-free plasma standardthe latter obtained by processing fresh plasma through a Sep-Pak C18 cartridge Millipore Corporation, Mildford, Massachussetts.
After incubation for 72 h at 4 C, one-hundred microliters of labelled Polipeptido pancreatico were added and the tubes incubated for polipeptido pancreatico additional 24 h.
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The mixture was incubated at C for 45 min and then centrifuged at g for 15 min at polipeptido pancreatico C. Finally, the pellet radioactivity was counted.
The intraassay and interassay coefficients of variation were 5. Pre-digestion of dietary lipids has only minor effects on absorption, retention and metabolism in larval stages of Atlantic cod Gadus morhua British Journal of Polipeptido pancreatico The hypothesis of the present study polipeptido pancreatico that cod larvae have a limitation in lipid digestion, and that absorption of lipids would increase by pre-hydrolysation.
polipeptido pancreatico The larvae were then incubated individually in chambers with collection of CO 2 for 10 h. No se usaron hormonas. Los islotes desarrollados se diferenciaron e identificaron en el momento del explante.