Aim: The objective of this work was to explore the inception, development, and chemistry of the Pap stain method introduced in by Dr. Introduction, Principle, Reagent Preparation, Procedure and Result Interpretation of both Progressive and Regressive Papanicolaou Stain or. Papanicolaou Stain for Cytological Preparations - Technical Memo METHOD: Fixation/Technique: Choice of fixative and technique is dependent on specimen.


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This is the first acidic counterstain cytoplasmic stain which stains matured and keratinized cells. The target structures are staine d orange in different intensities.

Papanicolaou - Histalim

This is the second counterstain which is a polychrome mixture of eosin Y, light green SF and Bismarck brown.

Four papanicolaou staining procedure were considered and scored background, cell morphology, nuclear staining, and overall staining pattern.


Fine needle aspiration cytology, giemsa, hematoxylin and eosin, modified ultrafast papanicolaou stain, papanicolaou stain Introduction Fine needle aspiration cytology FNAC is an accurate, low cost, and a rapid diagnostic test.

However, papanicolaou staining procedure speed of turnaround of test results varies among different institutions and in different clinical situations.

Modified ultrafast Papanicolaou staining technique: A comparative study

The speed of reporting of FNAC can be improved by rapid assessment of smears or on-site cytopathology. Quick diagnosis of FNAC plays an important role in efficient medical practice. In an outpatient setting, a quick report of aspiration cytology allows the clinician to discuss further lines of action or management options with the patient during the very first papanicolaou staining procedure, which is of great benefit for both the physician papanicolaou staining procedure the patient.

Then, the slides were washed in papanicolaou staining procedure tap water to remove the excess hematoxylin color. The slides were placed in EA for 45 s. After that, the slides were washed in absolute alcohol for six dips.


Finally, the slides were immersed in xylene for ten dips papanicolaou staining procedure mounted in DPX. Screening and assessment Background, nuclear staining, cell morphology, and overall staining were used as parameters to assess the quality of both staining methods. If the slide was fully papanicolaou staining procedure with red blood cells RBCsscore 1 was given.

When the slide contained a moderate number of RBCs, score 2 was given. If the slide was free of RBCs, score 3 was given. In thyroid slides, colloid also interferes with clarity of the background, so it was assessed in the same manner as with RBCs.

Cell morphology — Used to describe papanicolaou staining procedure cell shape.

Papanicolaou stain - Wikipedia

If the cells were well preserved and lacked any degenerative changes, they were given a score of 3. Applications Papanicolaou staining is commonly used to differentiate cells in such body fluids. The samples can be gynecological smear, sputum, urine, cerebrospinal fluid, papanicolaou staining procedure fluid… or all other samples containing cells.

  • Papanicolaou
  • Papanicolaou (PAP) Staining : Introduction, Principle, Procedure and Interpretation

Light Green SF yellowish stains the cytoplasm papanicolaou staining procedure other cells, including non-keratinized squamous cells. This dye is now quite expensive and difficult to obtain, therefore some manufacturers are switching to Fast Green FCF ; however, it produces visually different results and is not considered satisfactory by some.

Bismarck brown Y stains nothing and in contemporary formulations it is often omitted.